The Substance (2024) memes are inspired by Coralie Fargeat's satirical body horror masterpiece starring Demi Moore and Margaret Qualley. The film follows Elisabeth Sparkle, a fading star who takes a mysterious black-market drug to create a younger, more perfect version of herself named Sue. Online, the meme typically focuses on the 'The Substance' injection kit or the jarring transition between the two characters to highlight the gap between reality and expectation. It is widely used to joke about self-improvement, aging, and the horrific lengths people go to for social validation, often utilizing the 'Balance' and 'One Week' rules mentioned in the movie as comedic frameworks.