The 'What's My Necklace Made Of?' meme is derived from a scene in the medical drama series House M.D., featuring the cynical protagonist Dr. Gregory House. In the scene, House identifies that a patient's 'pearl' necklace is actually made of something far less glamorous, showcasing his diagnostic brilliance and signature lack of bedside manner. On the internet, the template is used to pivot from a seemingly innocent or beautiful statement to a harsh, often disgusting, or cynical reality. It serves as a perfect vehicle for 'well, actually' moments or subverting expectations with a blunt reveal, reflecting the character's abrasive but observant personality that fans of the show adore.