The 'Worst Mistake of My Life' meme originates from the Season 4 episode of Family Guy titled 'Petergeist,' which first aired in 2006. In the scene, Peter Griffin is seen running frantically out of a house while shouting the line in a state of pure panic. The meme gained massive popularity on platforms like Reddit, Twitter, and TikTok, where users apply it to situations ranging from accidentally clicking a cursed link to making a poor life choice. It has become a universal shorthand for instant, catastrophic regret in internet culture.