This iconic scene comes from the 2005 Family Guy episode 'The Courtship of Stewie's Father.' In an attempt to cheer up a depressed Peter Griffin (voiced by Seth MacFarlane), the family dog Brian (also voiced by MacFarlane) dons a banana costume and performs the famous 'Peanut Butter Jelly Time' dance. The sequence is a direct parody of the 2002 Flash animation featuring a pixelated banana, which was originally set to the song by the Buckwheat Boyz. The clip became a viral sensation in its own right, bridging the gap between early internet Flash culture and mainstream television. Today, it is used to represent mindless celebration, hyperactive energy, or the act of being intentionally annoying to a friend. Common captions include: 'Me when the pizza finally arrives,' 'My last two brain cells during a final exam,' and 'Trying to get my best friend's attention like.'