The 'Are You Challenging Me' meme features Shaggy Rogers from the 2011 animated film 'Scooby-Doo! Legend of the Phantosaur,' specifically during a scene where he enters a trance-like state of bravery. The meme gained massive traction in early 2019 as part of the 'Ultra Instinct Shaggy' trend, which reimagines the cowardly character as an omnipotent, god-like being capable of immense destruction. It is typically used as a reaction image to respond to someone making a boastful claim or issuing a challenge, suggesting that the responder is far more powerful than they appear. The humor lies in the absurdity of Shaggy being a serious threat, contrasting his usual persona with this intense, defiant expression.