The '3, 2, 1, Go!' meme originates from the rhythm-action game 'Rhythm Thief & the Emperor's Treasure,' released for the Nintendo 3DS. Specifically, it features the character Fondue, the protagonist's dog, barking along to a countdown during a mini-game. The audio clip became a viral sensation on platforms like TikTok and YouTube, where creators use the rhythmic buildup to time sudden transitions, comedic falls, or unexpected visual changes. It serves as a perfect auditory cue for high-energy content, capturing the nostalgic charm of the original game while providing a versatile framework for modern internet humor.